【摘要】 对培养人眼视网膜色素上皮（ＲＰＥ）细胞磷酸肌醇（ＩｎｓＰｓ）水平的分析表明，Ｃａｒｂａｃｈｏｌ显著刺激ＲＰＥ细胞ＩｎｓＰｓ水平的升高，且该效应可被阿托品所阻断，表明在入眼ＲＰＥ细胞存在毒蕈碱受体。去甲肾上腺素、５－羟色胺、表皮生长因子（ＥＧＦ）、异丙肾上腺素、和ＮＥＣＡ对ＩｎｓＰｓ基础水平无明显影响。异丙肾上腺素和ＮＥＣＡ对Ｃａｒｂａｃｈｏｌ刺激ＩｎｑＰｓ的效应也无明显影响；但ＥＧＦ显著促进Ｃａｒｂａｃｏｌ刺激人眼ＲＰＥ细胞ＩｎｘＰｓ，水平升高的效应。提示在人眼ＲＰＥ细胞ＥＧＦ与毒章碱受体之间存在有受体间交互作用。更多还原

【Abstract】 n this study iwe show that carbachol stimulates the accumulation of inositol phosphates(insps)in human retinal pigment epithelium (RPE)cells and atropine blocks the carbachol-induced effect,suggesting the existence of muscarinic acetylcholine receptors in human RPEcells. In contrast . noradrenaline. serotonin. epidermal growth factor (EGF) , isoproterenol. andNECA ( 5-[N-ethyl]-carboxamido-adenosine ) do not influence the basal levels of insps.Moreover. isoproterenol and NECA do not affect the carbachol-elevated levels of insPs.EGF.however ,does potentiate the carbachol stimulated elevation of Insps in a dose-depen-dent rnanner ,suggesting an interaction between EGF and muscarinic receptors in cultured hu-nian RPE cells.更多还原