【摘要】 测定了Ｍ－ＣＳＦ，Ｃ－ＣＳＦ，ＧＭ－ＣＳＦ，ＩＬ－３，ＩＬ－６，ＭＧＦ，ＬＩＦ和ＴＧＦ－β＿１８种重组人生长因子单个或不同组合对白血病细胞系Ｊ６－１，Ｊ６－２集落形成率的影响。单个因子除ＴＧＦ－β＿１和ＬＩＦ表现出明显抑制效应外，其它因子均有不同程度的刺激活性。而在双因子协同下，ＴＧＦ－β＿１除与Ｍ－ＣＳＦ协同外，仍表现出明显的抑制作用，表明ＴＧＦ－β是Ｊ６－１，Ｊ６－２细胞系重要的负调节因子。从三种因子组合结果看出Ｍ－ＣＳＦ是Ｊ６－１细胞必需的细胞因子。值得注意的是，ＴＧＦ－β＿１与Ｍ－ＣＳＦ的组合对Ｊ６－１细胞有很强的增殖刺激效应。另外由Ｊ６－１细胞膜分离出的膜相关细胞因子（ＭＡＦ－Ｊ６－１）单独和与ＴＧＦ－β＿１联合，对Ｊ６－１，Ｊ６－２细胞的作用与Ｍ－ＣＳＦ的作用相似更多还原

【Abstract】 ffects of recombinant human cytokines including M-CSF,G-CSF,GM-CSF,IL-3,IL-6,MGF,LIF andTGF-βon colony formation of human leukemic cell linesJ6-1 and J6-2 were studied.The various stimulating ac-tivities of all cytokines on these cell lines were found ex-cept TGF-β₁·and LIF,which showed significantly in-hibitory effects. In addition,TGF-β₁ when combinedwith other cytokines except M-CSF also possessed in-hibitory activity, and ihdicated that TGF-β₁ was an im-portant negative regulation factor for J6-1 and J6-2.Thesignificant stimulating effects of M-CSF on J6-1 wasfound even when it combined with TGF-β₁.It suggestedthat M-CSF play an important role on proliferation ofJ6-1.The effects of MAF（Membrane Associated Fac-tor）-J6-1 on J6-1 and J6-2 were similar to those of M-CSF when used alone or combined with TGF-β₁.更多还原