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急性髓细胞白血病M2b型AML1-ETO融合基因转录本的检测
DETECTION OF AML1-/ETO FUSION mRNA IN ACUTEMYELOGENOUS LEUKEMIA-M2b
【摘要】 急性髓细胞白血病(AML)-M_(2b)患者约90%有t(8;21)。已经证明它导致AML_1-ETO融合基因。我们应用逆转录酶/聚合酶链反应(RT/PCR)检测了22例AML_1-M_(2b)其中t(8;21)阳性16例,阴性2例,不能确定有无t(8;21)的4例,发现全部22例患者均可检出AML_1-ETO融合基因转录本。同时在5例t(8;21)阴性的AML中,包括M_(2a)2例,M_42例,M_31例,均未检出上述融合基因转录本。我们的初步结果提示AML_1-ETO融合基因可以作为AML-M_(2b)的基因标志。
【Abstract】 bout 90% of patients with acute myelogenousleukemia (AML)-M2b have the unique chromosomeabnormality, t (8; 21) reciprocal translocation. It hasbeen shown recently that t (8 ; 21) results in AML1-ETOfusion gene. By using reverse transcriptase-polymerasechain reaction (RT/PCR), the transcript of AML1-ETO fusion gene was assayed in 22 cases of AML1-M2bincluding 16 cases with t(8 ; 21), 2 without t(8 ; 21) and4 without karyotypic definition. The AML1-ETO fusionmRNA was detected in all cases studied. Parallel studyshowed that the transcript of AML1-ETO fusion genecould not be detected in 5 cases of t (8; 21 ) negativeAML including 2 cases ofM2b, 2 cases of M4[and 1 caseM3 . Our results indicated that AML1-ETO fusion genecould be regarded as a molecular marker of AML-M2b.
【Key words】 Acute myelogenous LeukemiaPolymerase chain reaction AML1-ETO fusion gene;
- 【文献出处】 中华血液学杂志 ,CHINESE JOURNAL OF HEMATOLOGY , 编辑部邮箱 ,1994年01期
- 【分类号】R733.71
- 【被引频次】15
- 【下载频次】113