【摘要】 急性髓细胞白血病（ＡＭＬ）－Ｍ＿（２ｂ）患者约９０％有ｔ（８；２１）。已经证明它导致ＡＭＬ＿１－ＥＴＯ融合基因。我们应用逆转录酶／聚合酶链反应（ＲＴ／ＰＣＲ）检测了２２例ＡＭＬ＿１－Ｍ＿（２ｂ）其中ｔ（８；２１）阳性１６例，阴性２例，不能确定有无ｔ（８；２１）的４例，发现全部２２例患者均可检出ＡＭＬ＿１－ＥＴＯ融合基因转录本。同时在５例ｔ（８；２１）阴性的ＡＭＬ中，包括Ｍ＿（２ａ）２例，Ｍ＿４２例，Ｍ＿３１例，均未检出上述融合基因转录本。我们的初步结果提示ＡＭＬ＿１－ＥＴＯ融合基因可以作为ＡＭＬ－Ｍ＿（２ｂ）的基因标志。更多还原

【Abstract】 bout 90% of patients with acute myelogenousleukemia （AML）-M_2b have the unique chromosomeabnormality, t （8; 21） reciprocal translocation. It hasbeen shown recently that t （8 ; 21） results in AML₁-ETOfusion gene. By using reverse transcriptase-polymerasechain reaction （RT/PCR）, the transcript of AML₁-ETO fusion gene was assayed in 22 cases of AML₁-M_2bincluding 16 cases with t（8 ; 21）, 2 without t（8 ; 21） and4 without karyotypic definition. The AML₁-ETO fusionmRNA was detected in all cases studied. Parallel studyshowed that the transcript of AML₁-ETO fusion genecould not be detected in 5 cases of t （8; 21 ） negativeAML including 2 cases ofM_2b, 2 cases of M₄[and 1 caseM₃ . Our results indicated that AML₁-ETO fusion genecould be regarded as a molecular marker of AML-M_2b.更多还原