【摘要】 自１９８９年以来，在冷冻保存异体血管移植研究基础上，开展冷冻异体血管化骨移植的实验研究与临床应用。首先对４只狗含后肋间血管的肋骨段采用两步冷冻保存法，慢降温（０．５℃～１．０℃／ｍｉｎ）至－６０℃，再投入－１９６℃液氮中保存４天，经快速复温后，施行显微血管吻合异体骨移植。术后经免疫学检测，ＳＰＥＣＴ扫描，血管造影及组织切片等显示移植骨血循环丰富，代谢活跃，３个月达骨性愈合，无排斥反应。于１９９２年３～８月为５例儿童因长管骨肿瘤术后骨大块缺损施行冷冻保存异体活骨移植，供体采取患儿父、母亲的髂骨瓣。经１０～１６个月随访，疗效满意。文中对克服排斥反应；重建移植骨血循环及手术适应证等进行探讨。更多还原

【Abstract】 AbstractSince 1989,an experimental study andclinical application of transplantation of cry-opreserved vascularized bone alfografts wascarried out in our hospital.The posteriorsegments of rib pedicled with intercostal ves-seis or four dogs were stored in liquid nitro- gen 4 days after sfowly cooled at 0.5～1.0℃/min to a holding temperature-60℃, and thenrapidly thawed before transplanted as allo- grafts.The grafts were analysed post-operativelyby immune response monitoring, SPECT scan-ning, angiography, and pathologic examina-tion,The results of the present study indica- ted that the grafts had good blood supplywith active metablism, and bone healing wasperfect at 3 months with no evidence ofimmunology rejection. From March to August1992,5 child cases of massive bone defectsresulted from resection of bone tumor weretreated by cryopreserved vascularized boneallografts. The donor iliac bones with deepcircumflex iliac artery were abtained frompatients’father or mother.Excellent functionrecovered after 10 to 16 months follow-uppost-operatively, The prevention of rejection. the reconstruction of blood circdatfon and theindication for such bone allografts are dis-cussed in this articale.更多还原