【摘要】 我院于１９９２年开始应用多聚酶链反应（ＰＣＲ）技术，对同种异体肾脏移植的供受者ＨＬＡ－Ⅱ类抗原进行基因分型。通过４０例患者基因分型结果表明：肾移植患者供受者有１～２个ＨＬＡＤＱＡ等位基因相符者，移植肾１年存活率１００％；完全不相符者，１年存活率３３％，两者有非常显著性差异（Ｐ＜０．００５）。采用此方法对器官移植供受者进行基因分型，可大大提高供受者间组织相容性相符程度，减少或减轻排斥反应的发生，以期提高移植物的存活质量，获得更好的临床效果。更多还原

【Abstract】 HLA-DQA allelt matching between the donor and recipient of kidney transplantation was performed with PCR technique since 1992 in our hospital. The results from 40 cases showed, if 1- 2 HLA- DQA alleles between donor and recipient was identical the one- year graft survivalwas 100%, if no allele compatible, the one-year graft survival was only 33% (P<0. 05). By means of DNA typing for HLA antigens, the donor-recipient compatibili-ty could be greatly improved, which resulted in fewer episodes of rejection and longer survival of the graft. This test was a quicker, more sensitive and specific technique which needed only little specimen, and was easy to repeat with accurate results.更多还原