【摘要】 用ＡＢＣ免疫组织化学方法，以抗Ｐ＿（５３）蛋白单克隆抗体ＰＡｂ＿（１８０１）为探针，研究了抑癌基因Ｐ＿（５３）产物Ｐ＿（５３）蛋白在７８例各型肺癌组织中的表达情况，发现Ｐ＿（５３）蛋白在肺癌组织中有较高的表达，总阳性率达８０．８％。在正常细胞未能发现突变Ｐ＿（５３），因而考虑Ｐ＿（５３）基因点突变是肺癌细胞的重要标志物。除文献报道的小细胞肺癌外，其他型肺癌也有很大一部分为细胞质阳性。提示在肺癌发生过程中Ｐ＿（５３）基因及产物的异常起着很重要的作用。更多还原

【Abstract】 he expression of tumour suppressor geneP,3products-P₅₃protein in patients with primary lungcancer has been studied bv ABC immunohistochemical method using McAb 1801 as probe. Ab-nornialities in P₅₃expression were found in 63 of 78carcinomas, 23 of 26 squamous cell carcinomas, 23 of29 adenocareinomas, 7 of 11 large cell carcinomas, 7of 9 small cell carcinomas and all 3 cases of adenoidcystic carcinomas showing abnonlial Ps3expression,whereas no expression of P₅₃ was deteetable in 11normal lung samples. These findings suggest that thepathogenesis of lung cancer may also be related withabnormalities of P₅₃ gene.更多还原