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紫外分光光度法测定维乐生片含量
Ultraviolet spectrophotometric assay for weilasheng tablet
【摘要】 采用紫外分光光度法测定维乐生片中维生素B1和维生素B6含量,以磷酸盐缓冲液(pH7.0)作溶媒,制成溶液在325nm波长处测得的A值为维生素B6的吸收度,吸收系数(E)为350;在268nm波长处测得的A值减去A325×0.232后的差值为维生素B1的吸收度,吸收系数(E)为245。回收率维生素B6为100.2%,RSD=0.3%;维生素B1为100.9%,RSD=0.9%。维生素B12含量测定按广东省标准法。
【Abstract】 he contents of vitamine B1 and B6 in the weilasheng tablet was determined by ultraviolet spectrophotometry.Phosphatebuffer(pH 7.0)was used as solvent. The absorbance of vitamme B6 was measured at 325 nm,its E1%1cm was 350,the ab-sorbance of vitamine B1 was the difference of A268 and 0.232 A325,theE1%1 cm was 245. The recovery of vitamine B6 was100.2%RSD=0.3%.and of vitamine B1,was 100.9%,RSD=0.9%.
【关键词】 维乐生片;
紫外分光光度法;
含量测定;
【Key words】 weilasheng tablet; ultroviolet spectrophotometry; content assay;
【Key words】 weilasheng tablet; ultroviolet spectrophotometry; content assay;
- 【文献出处】 中国医院药学杂志 ,CHINESE JOURNAL OF HOSPITAL PHARMACY , 编辑部邮箱 ,1994年06期
- 【分类号】R927.2
- 【下载频次】121