【摘要】 本文采用高效液相色谱法测定地黄中梓醇的含量。以 ＫＹＷＧ－Ｃ１８为固定相，０．６％乙腈为流动相，ＵＶ检测波长为２１０ｎｍ，梓醇进样量在２～１０μｇ与峰面积呈直线相关（ｒ＝０．９９９９；回归方程Ｙ＝０．０００７１５８＋０． ００００４３１８Ｘ）。平均回收率为 ９９． ００％，ＲＳＤ为 ３． ３８％（ｎ＝６）。并用该方法对河南产 １６批鲜地黄和生地黄样品进行了梓醇含量测定。更多还原

【Abstract】 Catalpol in radix rehmanniae was determination by high performance liquid chromatography. Catalpol was separated on a KYWG -C18 column (10um, 4. 6mm X 25cm), using water acetonitrile (99. 4: 0. 6) as mobile phase, and detection at 210nm. There was a good linearity ranging from 2 to 10ug of catalopl (r= 0. 9999, Y= 0. 0007158+0. 00004318X). The average recovery was 99. 00% and the coefficient of variation being 0. 5% (n= 6). Catalpol in 16 fresh radix rehmanniae and dry radix rehmanniae produced in Henan province was determined by this method.更多还原