【摘要】 对转“黄瓜花叶病毒外壳蛋白（ＣＭＶＣＰ）＋卫星ＲＮＡ”嵌合基因的工程ＮＣ８９的Ａ、Ｂ、Ｃ三个纯系以及转“ＣＭＶＣＰ＋烟草花叶病毒外壳蛋白（ＴＭＶＣＰ）”嵌合基因的工程ＮＣ８９Ｔ纯系进行了田间抗病性鉴定及农艺性状测试，结果表明：不论在人工接毒或自然发病条件下，转基因ＮＣ８９的花叶病病情指数都明显低于未转基因ＮＣ８９对照，其中Ｂ纯系的抗病性最高，在上述条件下，相对防效分别达到８０．２％、９２．９５％。在农艺性状方面，转基因ＮＣ８９较对照有所改善，但种性未变。更多还原

【Abstract】 The resistance to Cucumber Mosaic Virus and Tobacco Mosaic Virus and the preservation of cultivar properties in transgenic tobacco lines of a major commercial cv NC89 were tested under field conditions. Three of the transgenic lines, ddsignated A. B and C,possessed the chimeric genes of cucumber mosaic virus (CMV)satellite (Sat) RNA and coat protein (CP) and one of them, ddsignated T, possessed the chimeric genes of“CMV CP+TMV CP”. The results indicated that the transgenic lines showed much higher resistance than the non-transformed plants, and the“CMV CP+Sat RNA”-mediated protection was more effective than the“CMV CP+TMV CP”. -mediated protection. The disease indexes of the transgenic line B, which showed the highest resistance, averaged less than 3 and 6 in contrast to about 14 and 83. 7 of the non-transformed NC89 under natural conditions and with inoculation of viruses respectively. The cultivar properties of the transgenic lines improved but showed no genetic variation compared with the non-transformed plants.更多还原