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EDS-BH91毒株的分离鉴定

Isolation and Identification of EDS-BH91 Strain

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【作者】 胡子信; 张歧蜀; 门常平; 黄荣生; 崔德凤;

【Author】 Hu Zixin Zhang Qishu Men Changping(Department of Amimal Husbandry & Veterinary,Beijing Agriculture College)

【机构】 北京农学院牧医系; 北京农学院牧医系;

【摘要】 <正> 京效县一些种鸡和蛋鸡场暴发一种以发病率高、产蛋量大幅度下降(达30~60%)、蛋质量改变、产畸形蛋(增加到17%)为特征的疫病。将无菌病料接种鸭胚,分离到病毒,并命名为EDS—BH91。现将病毒分离和鉴定结果报告如下。 材料和方法 一、病毒分离 1.病料:从发病的不同鸡场取得3份材料(包括直肠、输卵管、卵巢、肝、脾、肾、气管、肺、气囊等)。

【Abstract】 The strain of Egg Drop Syndrome Verus (EDS-BH91V) was isolated by inoculating duck embryo with organs taken from the infected flocks with high infection and egg drop up to 30-60%,deformational eggs 17%. The isolate was propagated in duck embryo as well as duck em- bryo fibroblasts (DEF). It caused typical embryo-pathic effects and cytopathic effects (CPE). Electron microscope observation,test for susceptibility to chloroform ,Serological tests (HA&HI, cross-HI,AGP)and cross Immuno-protective test demonstrated that the siolate was similar to EDS-76-AV127strain in many aspects ,Such as antigenicity,morphology and other properties but caused defferent CPE in DEF. It belongs to the Aviadenovirus.

  • 【分类号】S852.65
  • 【下载频次】11
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