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灰茶尺蛾核型多角体病毒某些生化特性的研究
A Study on Some Biochemical Characteristics of Nuclear Polyhedrosis Virus of Ectropis grisescens warren
【摘要】 灰茶尺蛾核型多角体病毒某些生化特性的研究李彦章,石正丽,陈棣华(中国科学院武汉病毒研究所,武汉430071)关键词灰茶尺蛾,核型多角体病毒,结构多肽,限制性内切酶图谱灰茶尺蛾核型多角体病毒(Ectropisgrisescensnuclearpolyh...
【Abstract】 ctropis grisescens warren nuclear polyhedrosis virus(EgNPV)is a kind of mono-embeded bac-ulovirus.SDS-PAOE exhibits that the polyhedrin has only one major polypeptide band with MW of30.8kD.The virions have at least 18 polypeptide bands with MW ranged from 18kD to 105kD.Likeother insect NPVs,EgNPV polyhedrin also contains more Asp,Glu,less His,Met,and Cys This re-sult suggests that the genes of polyhedrin in many NPVs may have a conservative sequence.The DNA of EgNPV has been analyzed by REM with EcoRI,HindⅢ,aamHI,XhoI,EcoRI+BamHI,XHoI+EcoRI.The MW of every fragment has been determined,Total MW of EgNPV-DNAis 7.3×10 ̄6 Daltons.
【Key words】 Ectropis grisescens warren; Nuclear polyhedrosis virus; Stuuctural polypeptide; Restriction endonuclease map;
- 【文献出处】 中国病毒学 ,VIROLOGICA SINICA , 编辑部邮箱 ,1994年03期
- 【分类号】S476.13
- 【被引频次】4
- 【下载频次】41