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套式PCR检测婴幼儿巨细胞病毒感染

Detecti on of Human Cytomegalovirus in Neonatal Hepatitis by Nested PCR

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【作者】 单祥年陈金东严明鲁晓邱定红

【Author】 Shang Xiangnian;Chen Jindong;Yan Ming et al(Department of Biology,Nanjing Railway Medical College,Nanjing 210009

【机构】 南京铁道医学院生物教研室

【摘要】 本文采用套式PCR(Nestedpolymerasechainreaction)技术对61例婴儿肝炎综合征(Neonatalhepatitis)患者进行了HCMV(Humancytomegalovirus)检测,结果表明,当只用一对引物进行PCR时,检出33例患者为HCMV阳性,阳性检出率为54.1%;而采用套式PCR后,有17例为HCMV阳性,阳性检出率提高到77.0%。可见套式PCR方法的阳性检出率明显高于单一PCR,并远高于目前使用的其它方法。同时也说明HCMV是导致肝炎综合征的主要病原体之一。此外,灵敏性和特异性试验表明,套式PCR技术的敏感度高、特异性强、简单快速,在临床检测和诊断中具有很大的潜力。

【Abstract】 ixty-one children patients with neonatal hepatitis were investigated for human cytomesalovirus(HCMV)by single PCR and nested double PCR.of the 61 patients,33(54.1%)were positive forHCMV by single PCR,whereas 47(77.0%)were positive by nested double PCR.This showed that nest-ed double PCR is more sensitive than single PCR,and also revealed that HCMV can be one of the important agents for neonatal hepotitis.In addition,its advantage of high sensitivity and specificty com-bined with more rapid detection suggests that this precedure can be a potential diagnostic and epidemio-logical tool.

  • 【文献出处】 中国病毒学 ,VIROLOGICA SINICA , 编辑部邮箱 ,1994年02期
  • 【分类号】R725.129.9
  • 【下载频次】25
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