【摘要】 建立了用ＨＰＬＣ柱切换测定血浆中氟康唑的方法。血样用０．２ｍｏｌ·Ｌ－１醋酸溶液简单稀释后注入填充ＬｉＣｈｒｏｍｐｒｅｐＲＰ２（２５～４０μｍ）的预柱上，用蒸馏水冲洗出血浆中蛋白和其它极性成分。切换后，浓缩在预柱上的氟康唑被流动相甲醇－０．２ｍｏｌ·Ｌ－１醋酸按（ｐＨ２．７）（５０：５０）反冲到分析柱ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ上进行分析。预柱用净化液进行清除和再生。本法有很好的精密度与回收率，检测限为０．１２μｇ·ｍｌ－１血浆，日间和日内测定相对标准偏差均小于６％。此分析法已成功地用于测定自愿受试者的氟康唑药代动力学及生物利用度。更多还原

【Abstract】 High performance liquid chromatography with colunm switching has been developed for the determination of fluconazole in plasma.The plasma samples were injected onto a precolumn packed with LiChromprep RP2(25～40μm)after simple dilution with a solution of 0.2 mol·L-1 acetic acid.Distilled water was used to wash out protein and other polar components in plasma.After switching, the concentrated fluconazole was eluted in the back-flush mode onto a Shimpack CLC-ODS column with methanol-0.2 mol·L-1 ammonium acetate(pH 2.7)(50 :50 )as mobile phase.Purge solutions were used for clean-up and for regenerating the precolumn,The method showed a good precision and excellent recovery.The detection limit was 0.12μg·ml-1 plasma.The RSD’s(intra-andinterday )were less than 6.3%. The method has been successfully used to determine bioavailability and pharmacokinetics of fluconazole in Chinese volunteers.更多还原