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TC-1基质细胞系对造血干、祖细胞的刺激作用

STIMULATION OF TC-1 STROMAL CELL LINE ON HEMATOPOIETIC STEM CELLS AND PROGENITOR CELLS

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【作者】 高春记何一心任红光宋增璇

【Author】 Chun-Ji Gao(Department of Hematology, Chang Cheng Hospital Beijing 100853)Yi-Xin He Hong-Guang Ren Zeng-Xuan Song (Institute of Hematology, Chinese Academy of Medical Sciences Tianjin 300020)

【机构】 长城医院血液科中国医学科学院血液学研究所中国医学科学院血液学研究所 北京 100853天津 300020天津 300020

【摘要】 TC-1基质细胞系是从小鼠骨髓细胞长期液体培养中分离出来的细胞系,其条件培养液中具有多种生物活性物质,被认为多因子分泌的细胞系。TC-1条件培养液与正常小鼠骨髓细胞共同孵育,然后通过粒、单系祖细胞(CFU-GM)半固体培养试验,脾结节形成试验,放射保护试验及流式细胞仪测定骨髓细胞的周期特点,证实了TC-1细胞系能分泌粒、单系集落刺激活性物质(GM-CSA)及体外对多能造血干细胞具有保存作用。

【Abstract】 A murine marrow stromal cell line, termed TC-1, secretes various hematopoietic growth factors. Incubation of fresh murine bone marrow cells with TC-1 conditioned medium (CM) in liquid culture resulted in a marked stimulation of hematopoietic cell proliferation. After 24h of culture, there were increased total cell numbers and blast cells in the cell suspension with TC-1 CM compared with the control group. CFU-GM content increased by 18% by 24h of culture with TC-1 CM. In contrast, in the absence of TC-1 CM only 40% CFU-GM remained. The number of day 12 CFU-S (CFU-S12) also increased in the group with TC-1 CM for 24h (No cells: CFU-S12<50; Fresh bone marrow cells: CFU-S12 13.30+1.20/5 × 104MNC; Bone marrow cells treated with TC-1CM: CFU-S12 14.30+1.74 / 5 ×14MNC; Bone marrow cells incubated with culture medium, without TC-1 CM: CFU-S126.10+0.68). The radioprotection assay results showed that the 30 days survival rate was 60-70% when the lethally irradiated mice were injected with 1 ×106 fresh bone marrow cells or bone marrow cells incubated with TC-1 CM for 24h. In comparison, only 20% of the mice survived in the group injected with cells incubated in culture medium without TC-1 CM. Cell cycle analysis of bone marrow cells was performed by flow cytometry. The percentage of S-phase and G2M-phase cells in bone marrow cells incubated with TC-1 CM increased. Taken together, these data indicated that TC-1 cell line may also secrete factors effecting hematopoietic stem cell survivalin addition to CSF.

  • 【文献出处】 中国实验血液学杂志 ,Journal of Experimental Hematology , 编辑部邮箱 ,1994年01期
  • 【分类号】R329
  • 【被引频次】1
  • 【下载频次】30
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