【摘要】 利用免疫印渍技术分析杜氏利什曼前鞭毛体蛋白分子量,发现有3条明显的蛋白带(63kDa、55kDa和50kDa).用这3条蛋白带分别和黑热病人血清及其它血清进行酶联免疫印渍试验,63kDa蛋白带的灵敏度为100.0%,特异性为99.0%,误诊率1.0%,漏诊率0.0%,阳性预测值92.0%,阴性预测值100.0%,均较55kDa和50kDa蛋白带实验结果为好.鉴于该实验方法简单,结果可靠,值得进一步研究.更多还原

【Abstract】 Protein of leishman promastigotes were analysed with immunoblotting,and three bands of protein were found(63kDa、55kDa、50kDa),with these three bands,sera of 11 kala-azar patients and other 112 non-infected persons were tested with ELIB. We found the protein band of 63 kDa showed better antigenicity. The sensitivity,specificity, false positive,false negative,positive predicative value and negative predicative value were 100. 0%,99. 0%,1. 0%, 0. 0%,92.0% and 100. 0% respectively. The results were better than that of 55 kDa and 50 kDa. Owing to the fact that this test is easier to carry out and posseses better results,it is re-ommanded for further large scale study.更多还原