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火鸡隐孢子虫在鹌鹑体内发育史的研究

THE LIFE CYCLE OF CRYPTOSPORIDIUM MELEAGBIDIS IN QUAILS

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【作者】 查红波蒋金书

【Author】 Zha Hongbo; Jiang Jinshu (Coilege of Veterinary Medicine, Beijing Agricultural University 100094)

【机构】 北京农业大学兽医学院

【摘要】 分离自鹌鹑的火鸡隐孢子虫(Cryptosporidiummeleagridis)经口感染2日龄鹌鹑,潜在期(Prepatentperiod)为36,开放期(Patentperio)为4~18d。它在鹌鹑体内发育史过程为:卵囊经口接种后,于十二指肠、空肠、回肠肠腔中子孢子脱囊,至接种后6h全部子孢子脱囊完毕。接种后6h在小肠中出现滋养体(大小为3.16μm)。接种后12h在小肠中出现内含8个裂殖子的裂殖体(大小为4.61×3.96μm)及裂殖子(大小为4.32×0.952μm),接种后48h滋养体和裂殖体开始出现于直肠。接种后54h,在小肠、直肠中出现含有16个子弹形小配子的小配子体(大小为3.41×3.30μm)。小配子(大小为0.872×0.452μm)与大配子(大小为4.61-×4.35μm)结合,形成合子。接种后72h出现未孢子化的厚壁型卵囊。滋养体、裂殖体、大配子、小配子体、厚壁型卵囊均处于宿主肠绒毛上皮细胞的带虫空泡之中。厚壁型卵囊于带虫空泡中孢子化。本文是首次有关C.meleagridis发育史的完整和详尽的报道。

【Abstract】 The prepatent period for Cryptosporidium meleagridis was 3 days post oral inocula-tion (PI) of oocysts isolated from quails (Coturnix coturnix), and the patent periodwas 4-18 days PI for the quails inoculated at 2 days of age. The life cycle of Crypto-sporidium melegaridis in quails is described as follows.Excystation that sporozoites were released froni oocysts occurred in the lunien ofduodenum, jejunum, ileum 2-4h PI and no oocysts of oral inoculation existed 6h PI.Trophozoites (measured 3.16μm) first appeared in the microvillous region of enterocytes(MRE) of the small intestine 6h PI. Mature meronts (4.61×3.96μm) with 8 merozoitesfirst appeared in tlie MRE of the small intestine 12h PI. Trophozoites and maturenieronts first appeared in the MRE of colon 48h PI. In the MRE of the small intestineand colon 54h PI first appeared microgamonts (3.41×3.30μm) with 16 microgametes.Released microgametes(0. 872×0. 452μm) Penetrated into macrogametes (4.61×4.35μm)to form zygotes. Unsporulated oocysts with thick-walls first appeared 72h PI. Trophozoi-tes, mature meronts, macrogametes, microgamonts and thick-walled oocysts were foundin the parasitophorous vacuoles (PV) of the MRE. Thick-walled oocysts were sporulatedin the PV. No thin-walled oocyst was found in all the study.

【关键词】 鹌鹑火鸡隐孢子虫发育史
【Key words】 QuailCryptosporidium melegaridisLife cycle
  • 【文献出处】 畜牧兽医学报 ,ACTA VETERINARIA ET ZOOTECHNICA SINICA , 编辑部邮箱 ,1994年03期
  • 【分类号】S858.395.9
  • 【被引频次】41
  • 【下载频次】64
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