【摘要】 本研究建立了自患者粪便中直接进行ＥＴ－ＨＥＶ核酸特异片段扩增的方法．即便经反转录反应后的ＰＣＲ放大法。ＰＣＲ流程为：变性：９４℃９０ｓ，退火：４０℃９０ｓ，延伸；７３℃１２０ｓ，共２５个循环。所用引物为ＥＴ－ＨＥＶ特异．从而决定了ＰＣＲ产物的特异性．该产物已用于制备ＥＴ－ＨＥＶＲＮＡ持异的诊断探针．更多还原

【Abstract】 A method,which was applied to the direct amplification from the feeal samples for the specific ET-HIV derived fragment, had been set up, by using PCR (Polymerase Chain Reaction ) right after reverse transcription. The PCR protocol was with 25 cycles of reaction through melting at 94℃ for 90 seconds, annealing at 40℃ for 90 seconds and extension at 73 ℃ for 120 seconds. The ET-HEV specificity of the primers used in this study resulted in that of the PCR amplification product,which had been used in the preparation of the diagnostic probes for ET-HEV RNA.更多还原