【摘要】 从病虾中制备病毒粗提液感染日本对虾健康成虾．实验在２０～２４℃水族缸中进行，感染途径分别为头胸甲内近肝胰脏处注射病毒粗提液、投注射病毒粗提液的新鲜牡蛎及直接投病虾肝胰脏．感染７ｄ后死亡率分别为５３．３％、５０％及５５％．利用电镜可在感染致死的对虾肝胰脏中观察到与作为感染源的病虾中形态、大小相同的病毒颗粒．结果表明该病毒可通过饵料由消化道传播，这种病毒感染方法可作为该种病毒人工传代培养的有效方法．更多还原

【Abstract】 Viral extraction prepared from sick Kuruma shrimps infect healthfuladult of Panaeus japonicus Bate. Three infection methods are taken : feeding oysters injectedwith viral extraction; injecting viral extraction to cephalothorax near hepatopancreas ofshrimps and feeding hepatopancreas of sick Kuruma shrimps. The infected shrimps are cul-tured at 20～24℃ in rearing jars. The mortality is 50%,53.3%and 55%during 7 days afterinjection respectively. It could be observed that the morphe of viruses of experimental infec-tion shrimps are the same as the sick shrimps of infective origin by electron microscope. It isdemonstrated that this virus could spread by digestive trace and that these infection methodscould be used for artificial propagation of this virus.更多还原