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玉米穗粒腐病接种技术及品种抗病性鉴定研究

Inoculating Technique of Corn Ear Rot and Identifying Resistance Varieties

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【作者】 李亚玲; 马秉元; 李多川; 龙书生;

【Author】 Li Yaling Ma Blngyuan Li Duochuan Long Shusheng(Shaanxi Institute of Plant Protection,SAAS,Yangling,Shaanxi, 712100)

【机构】 陕西省农科院植保所;

【摘要】 以玉米穗粒腐病的主要高原菌串珠镰刀菌FusariummoniliformeSheld为菌源,采用4种接种方法比较结果,以针刺果穗较深处(籽粒与穗轴间)发病最重,花丝喷雾法发病最轻;接种时期以乳熟期接种为好;接种浓度以10×40倍,每视野60个分生孢子接种效果最好;接种量以每果穗接种2.5mL孢子悬浮液发病重,效果好。

【Abstract】 Four inoculating methods with Fusarium moniliform Sheld as the mainpathogens of corn ear-rot were compared.The results indicated that using needle punc-turing corn ear produced the serious disease occurence,but the method of spraying ino-culation to filaments caused lightest disease occurence. The optimal inoculation time wasat milk stage. Among three inoculation concentrations,the most effective inocuation wasobtained by concentration of 60 conidia in one field of vision under microscope at 10 ×40times amplification. Comparative study of three amounts of inoculating,the sever diseaseand better effect were obtained by inoculating2.5mL conidial suspension to each corn ears.

【基金】 陕西省科委资助课题
  • 【文献出处】 西北农业大学学报 ,ACTA UNIVERSITATIS AGRICULTURAE BOREALI-OCCIDENTALIS , 编辑部邮箱 ,1994年01期
  • 【分类号】S513.034
  • 【被引频次】16
  • 【下载频次】184
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