【摘要】 ７．５～６０ｍｇ／Ｌ卡介苗（ＢＣＧ）刺激的肺泡巨噬细胞（ＰＡＭ）培养上清液能显著促进肺成纤维细胞（ＰＦＢ）增殖和胶原合成。发现该上清液中含较高水平的肿瘤坏死因子（ＴＮＰ），且其促ＰＦＢ增殖作用与ＴＮＦ水平成正相关，提示ＢＣＧ在体外能刺激ＰＡＭ产生ＴＮＦ，ＴＮＦ为该上清液促ＰＦＢ增殖的主要因素；５０ｍｇ／ＬＳｉＯ２刺激的ＰＡＭ上清液亦能促进ＰＦＢ增殖和胶原合成，但上清液中几乎检测不到ＴＮＦ，说明其促ＰＦＢ增殖和胶原合成的成分是不同于ＴＮＦ的其它物质；ＴＮＦ能协同该物质促ＰＦＢ增殖和胶原合成。上述结果提示，结核菌刺激ＰＡＭ产生ＴＮＰ可能是矽肺结核病较单纯矽肺发展迅速的一个重要原因。更多还原

【Abstract】 The supernatant of pulmonary alveolar macrophages (PAM) stimulated by 7.5～60mg/L of BCG could significantly enhance pulmonary fibroblasts (PFB) proliferation and synthesis of collagen. The supernatant contained high level of TNF. These results indicate BCG could stimulate PAM to produce TNP. On the other hand, supernatant of PAM stimulated by 50mg/L of SiO2 could also enhance PFB proliferation, but the level of TNF in this supernatant was very low. It demonstrated the ingredient in the supernatant that could enhance PFB proliferation was not TNF. TNF showed synergism with this ingredient for enhancing PFB proliferation. TNF produced by tuberculosis bacteria-stim ulated PAM might be a factor enhancing the development of fibrosis of silicotuberculosis.更多还原