【摘要】 <正> 1978年3月,作者首先发现了菜白蝶的一种新病毒.其后对该病毒的分离、鉴定、病毒的生物物理性质、血清学性质以及病毒的核酸性质等进行了报道.以其主要特性证明,该病毒可能为细小病毒科、密核病毒属的一个新种.Kusetak曾报道细小病毒科的密核病毒属,其病毒易感染脊椎动物的多种细胞.菜白蝶的这种新病毒是否具有此特性,作者用地鼠肾细胞进行了感染试验,现将结果报道如下.1 材料和方法1.1病毒增殖取低温保藏的毒种,置于常温下进行温度平衡.然后将毒种悬液涂于新鲜洁净的甘蓝叶上,饲喂经饥饿处理的菜青虫,感染5天左右,收集典型症状病死的幼虫,用无菌水研磨均匀,4000g×离心30分钟,除去虫体碎片,上清液再次扩大饲喂幼虫,如此反复至病死幼虫够量.更多还原

【Abstract】 In 1978,the first observation a New densovirus from pieris rapae was made by F. L. Sun. The virus is very small and have very high virulence. After 1989,we report their every character, example: biology, biophyics serology morphology and nucleat acid specificity. Those special were reported, but, how is the cell culture character? So we study the infectivity that infect cell of vertebrate. The virus all character are similar densovirus of parvoridae. Conseguently we suppose this viurs of pieris rapae is a New insect virus.更多还原