【摘要】 本文对香菇（Ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ）单核菌丝９１０１（１２）原生质体制备后的再生条件，进行了探索。结果表明，原生质体经过合０．６Ｍ．甘露醇的麦芽糖酵母粉培养基（ＭＹＧ）液体培养５天，用双层培养法，在含０．６ＭＭｇＳＯ４的高渗ＭＹＧ平板上经再生，再生率达５×１０－５。本文将Ｋｎｏｗｌｔｏｎ等于１９８４年创造的一种分离高频再生衍生株的方法首次运用至食用菌中，所得９１０１（１２）再生株Ｒ３，再生率提高２～３倍，为香菇原生质体融合操作打下基础。更多还原

【Abstract】 Conditions for regenerating the protoplasts from monoaryotic mycelium 9101 (12) were defined. The protoplasts were cultivated in an MYG medium for five days and then regenerated in the hypertonic MYG medium containing 0. 6M MgSO4, thereby the regeneration frequency being raised to 5×10-5, Furthermore, the method developed by Knowlton et al. for isolating high-regeneration-frequency strains was for the first time applied to the edible mushroom Lent inns edodes, By using this method we obtained a regeneration strain of R3, the regeneration frequency of which is 2-3 tinies that of monokaryotic mycelium 9101 (12).更多还原