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艰难梭菌荚膜多糖单糖组成的气相色谱分析
ANALYSIS FOR THE COMPOSITION OF MONOSACCHAR-OSE IN THE CAPSULAR POLYSACCHARIDE OF CLOSTRIDIUM DIFFICILE
【摘要】 本文对两株艰难梭菌(CdA318和CdCDC279)荚膜多糖进一步分析,结果证实纯化的艰难梭菌荚膜多糖为单一均匀的物质;经气相色谱分析表明,其单糖组分主要由D-葡萄糖、D-甘露糖和D-半乳糖组成,不同菌株的荚膜多糖可能有微小差异。
【Abstract】 The capsular polysaccharide(CPS)of two strains of Clostridium difficle(Cd)were pu-rified by Acetone precipitation.The CPS was identified homogeneous by Sepharose 4B columnchromatography and cellulose acetate film electrophoresis.According to the results of gas chro-matography;the CPS of Cd is mainly composed of D-glucose,D-mannose and D-galactose.
【关键词】 艰难梭菌;
荚膜多糖;
气相色谱;
【Key words】 Clostridium difficile; Capsular polysaccharide; Gas chromatography;
【Key words】 Clostridium difficile; Capsular polysaccharide; Gas chromatography;
【基金】 国家自然科学基金
- 【文献出处】 微生物学通报 ,MICROBIOLOGY , 编辑部邮箱 ,1994年04期
- 【分类号】R378.2
- 【被引频次】1
- 【下载频次】120