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体外培养新生大鼠心房壁中的CGRP-IR和NPY-IR神经细胞
In Vitro Culture of Calcitonin Gene-Related Peptide and Neuropeptide Y Immunoreactive Neurones from Atrial Wall of Newborn Rats
【摘要】 对体外培养新生SD大鼠心房壁的神经细胞,按SternbergerPAP法进行了降钙素基因相关肽(CGRP)和神经肽Y(NPY)免疫细胞化学研究。结果显示:CGRP-IR阳性细胞数量较少,实验的5份标本只有2份见到阳性细胞,而NPY-IR阳性细胞在所有实验标本中均可见到。本研究在培养条件下证实心内神经节中有CGRP-IR阳性细胞存在,支持心脏有器官内感觉神经细胞的设想。同时对心内神经细胞的化学性质进行了讨论。
【Abstract】 Intrinsic neurones of artia1 wall from newborn SD rats cultured in vitro were-studied by using calcifonin gene-related peptide(CGRP)and neuropeptide Y(NPY)immunocytochemical reaction according to Sternberger PAP method.Results demonstrated that Only few CGRP-IR cells were found.Positive cells were found in two of five samples,while PY-IR cells were found in all preparations examined.Present znvestigation has veri-fied the presence of neurones immunoreactive for CGRP in intracardiac ganglion in culture.Our results support the supposition that there are intrinsic sensory neurones within atrial wall.The chemical properties of ntracardiac neurone was also discussed.
【Key words】 cells; cultured; intracardiac neurone; calcitonin gene-related peptide; neuropeptide Y; immunocytochemistry;
- 【文献出处】 同济医科大学学报 ,Acta Universitatis Medictnae Tangji , 编辑部邮箱 ,1994年04期
- 【分类号】R329.2
- 【被引频次】4
- 【下载频次】33