【摘要】 建成小鼠胸腺基质细胞系,命名为MTSC4,经鉴定分析认为是DC来源。在无外来刺激条件下,MTSC 4细胞可自发分泌多种类型细胞因子,已检测到的有IL-1,IL-6,IL-7,CSF,IFN及趋化因子(CF)等。其中IL-6,IFN为较高水平分泌量,IL-1,CF为中等水平,IL-7、CSF为较低水平分泌量。MTSC4不能自发分泌IL-3及TNF_α。MTSC4的建立有利于分析胸腺选择特别是阴性选择的机理,及细胞因子在其中的作用,也可用于研究自分泌因子的网络调节。更多还原

【Abstract】 The cytokine production by MTSC 4 cells, a murine thymic dendritic cell line established in our laboratory, has been investigated. Without exogenous stimulation, the MTSC 4 cells constitively produce multiple types of cytokines, including IL-1, IL-6, IL-7, CSF, IFN and Chemotactic factor (CF). Of which, IL-6 and IFNwere abundant; IL-1 and Chemokine, moderate: IL-7 and CSF at low level. MTSC4 cells could not produce detectable either IL-3 of TNFα. The establishment of MTSC 4 may be useful in the analysis of the mechanism of thymus selection, and in the study of the network regulation of au-tocrined cytokines.更多还原