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食管癌细胞核基质蛋白电泳行为的分析

Analysis of Electrophoresis pattern of Nuclear Matrix proteins in Esophageal Carcinoma

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【作者】 钟叔平温博贵

【Author】 (Department of Biochemitry, Shantou University Medical College) Zhong Shu-ping et al

【机构】 汕头大学医学院生物化学教研室

【摘要】 以Robinson法制备食管癌细胞核基质,用10%分离胶,4%浓缩胶进行SDS-PAGE分离。结果表明,食管癌细胞核基质在40KD~60KD区间含有五条浓染蛋白区带,这五条核基质蛋白在食管癌细胞核中同样出现,但含量较低,这表明它们是一组富集的核基质蛋白。

【Abstract】 The nulear matrix of esophageal carcinoma was prepared according to the method of Robinson, SI. The nuclear matrix was electrophoresed with 40% running gel and 4% stacking gel. The result showed that the five protein bands were consisted of nuclear matrix of esophagral carcinoma between 40KD-60KD. The five protein bands were stained deeper in the nuclear matrix than in the nuclei of esophageal carcinoma. They would be characteristic proteins of nuclear matrix enriched.

【关键词】 食管癌核基质蛋白质
【Key words】 Esophageal CarcinomaNuclear MatrixNuclear Proteins.
  • 【文献出处】 汕头大学医学院学报 ,JOURNAL OF SHANTOU UNIVERSITY MEDICAL COLLEGE , 编辑部邮箱 ,1994年01期
  • 【分类号】R34
  • 【下载频次】17
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