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男性精液生殖细胞精蛋白的高效液相色谱分离研究
Study on Separation of Protamines in Human Sperm Cells by High Performance Liquid Chromatography(HPLC)
【摘要】 男性精液生殖细胞精蛋白的高效液相色谱分离研究刘智敏,何才云(华西医科大学分析测试中心成都610044)1前言在不育夫妇中,男性所致者约占50% ̄[1],精液异常是主要的原因之一 ̄[2],国内外对精液生殖细胞精蛋白的分离研究,已证明精蛋白异常是男性不育...
【Abstract】 PLC has been successfully used to the separation of protamines in sperm cells of fertile and infertilemen. The separation was carried out with TSK G3000 SW column,2mol/L ureaand UV detector at 216nm. From these chromatographic tests here it is shown that there haven’t been enough protamines in sperm cellsof infertile men by compared with fertile men. And this method can be used for assay of protamines in spermcells and is proved to be simple,accurate and sensitive.
【关键词】 高效液相色谱;
精蛋白;
人精液细胞;
【Key words】 high performance liquid chromatography; protamine; human sperm cell;
【Key words】 high performance liquid chromatography; protamine; human sperm cell;
- 【文献出处】 色谱 ,CHINESE JOURNAL OF CHROMATOGRAPHY , 编辑部邮箱 ,1994年06期
- 【分类号】Q592.903
- 【被引频次】1
- 【下载频次】59