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高效液相色谱溶剂系统四面体优化法
The Tetrahedron Method of Optimizing Solvent System in High Performance Liquid Chromatography
【摘要】 改进了Glajch的三角形法,建立了适合于高效液相色谱(HPLC)的四面体优化法。将7个溶剂系统的实验结果输入计算机,由计算机模拟231个溶剂系统的色谱行为,优选出最佳溶剂系统。将此法用于12种磺胺药物的分离,得到了满意的结果。
【Abstract】 n this paper we modified the triangle method proposed by Glajch and developed the tetrahedron method.In this method,after the experimental results of 7 solvent systems were input into a micro-computer,the latter simulated the chromatographic behaviour of 231 solvent systems and gave the optimal system. The tetra-hedron method has been successfully applied to the separation of 12 sulfadrugs.
【关键词】 高效液相色谱;
流动相的优化;
四面体优化法;
磺胺类药物;
【Key words】 high performance liquid chromatography; mobile phase optimization; the tetrahedron method; sulfa drugs;
【Key words】 high performance liquid chromatography; mobile phase optimization; the tetrahedron method; sulfa drugs;
- 【文献出处】 色谱 ,CHINESE JOURNAL OF CHROMATOGRAPHY , 编辑部邮箱 ,1994年05期
- 【分类号】O657.72
- 【被引频次】7
- 【下载频次】173