【摘要】 高效液相色谱法分离纯化人胎盘泌乳素酶切片段杨煜，杨波，麦荫乔（白求恩医科大学长春１３００２１）１前言人胎盘泌乳素（Ｈｕｍａｎｐｌａｃｅｎｔａｌｌａｃｔｏｇｅｎ，ＨＰＬ）是由胎盘绒毛滋养层的合体细胞分泌的一种单链多肽类激素。它主要有催乳作用￣［１］及促...更多还原

【Abstract】 n this paper, we present a simple isolation and purification method to get human placental lactogen fromfresh normal term human placenta. Some fragments were obtained by the cleavage of the molecule with crys-tal pepsin,and subjected to further purification by gel fiItration over a column of Sephadex G-50.After that,”with HPLC fractions for each peak were collected by using an LKB LiChrosorb RP 18( 4.0mm i.d×250mm)column at a flow rate of lmL/min and a pressure of l.4 × 107pa。The elution was operated with alinear gradient from 30%to 70%acetonitrile in 0.0lmol/Lammonium acetatesolution(pH 4. 0). RIA withradiolabelled ￣(125)I-HPL and￣(125)I-HGH was used for the determination of the immunoactivity of HPL in eachpurification steps and an enzymed fragment with high purification and specific activity yielded.更多还原