【摘要】 用ＴＰＰＳ＿４作柱前衍生试剂，寻找到试剂与锌（Ⅱ）和铜（Ⅱ）的最佳反应条件，然后在ＯＤＳ柱上，以乙腈－水体系（２４：７６，ｖ／ｖ）作流动相，四乙基碘化按作离子对试剂，在４１５ｎｍ处检测，确立了离子对高效液相色谱－光度法快速分离测定痕量锌和铜的新方法，检测下限（×ｌ０￣（－９）ｇ／ｍＬ）：Ｚｎ０．１０，Ｃｕ０．０９。所建立的方法用于花生样品中锌和铜的测定，结果令人满意。更多还原

【Abstract】 nly by heating at pH 4.4 chelating reaction of meso-tetra（4- sulfophenyl）porphyrin（TPPS₄）with Zn（Ⅱ）and Cu（Ⅱ） can be completed. ZnTPPS₄, TPPS₄ and CuTPPS₄ were separated within 8 min on an ODScolumn and eluted with acetonitrile+water（24 : 76,V/V）containing 50 mmol/L tetraethylammonium iodideand l0 mmol/L acetic acidsodium acetate buffer solution（pH 6, 4）, and detected at 4l5 nm。 The detectionlimits were 0.l and 0. 09（×10￣（-9）g/mL）for Zn and Cu respectively. This method can be used for the deter-mination of trace Zn and Cu in the peanut sample.更多还原