【摘要】 本文报道了使用米根霉（ＲｈｉｚｏｐｕｓＯｒｙｚａｅ）ＴＬ－５２７－９菌株用玉米淀粉深层发酵生产Ｌ－乳酸。探讨了不同碳源、氮源、底物浓度、液化条件、通气量、种龄对Ｌ－乳酸生成的影响，在３Ｍ３发酵罐中，当玉米淀粉投料浓度为１３％时，７罐平均产Ｌ－乳酸９．４１ｇ／１００ｍｌ，对糖转化率７９．５５％。发酵液经分离提取精制，Ｌ－乳酸纯度达９８％以上。同时在研究过程中利用高压液相色谱测定了Ｌ－乳酸代谢变化。本结果表明米根霉ＴＬ－５２７－９菌株具有Ｌ－乳酸产率高和发酵快的特点。更多还原

【Abstract】 This paper was to describe the production of L-lactic acid from corn starch by submerged fermentation method using Rhizopus oryzae TL-527-9. The infulences of different carborn sources, nitrogen sources,substrate concentration, liquification, aeration, inoculum age on the formation of L-lactic acid were ivestigated. If the concentration of corn starch used in the test was 13. 0% (W/V) ,the yield of L-lactic acid averaged 9. 41g/100ml in a 3M￣3 fermented after 7 batch with 79. 55 g of L-lactic acid obtained from per 100g sugar. After refining fermented broth the purity of L-lactic acid was over 98% .Otherwise ,the change of L-lactic acid metabolism was determinated with HPLC. The results indicated that the strain of Rhizopus oryzae TL527-9 was preferred on account of the much more yield of L-lactic acid production and shorter fermentation.更多还原