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酶促水解大豆分离蛋白的研究
Study on the Enzymic Hydrolysis of Soy Isolated Protein
【摘要】 本研究利用大豆分离蛋白为原料,通过正交设计试验,确定了碱性蛋白酶的适宜水解条件为:T=55℃,pH=8.0,S=10%,E/S=4%,并得到了收率高、水溶性好的水解蛋白。氨基酸组成表明水解蛋白中除含硫氨基酸外,其它六种必需氨基酸有较好的平衡,与NAS(1980)及FAO(1973)的参考模式相接近。
【Abstract】 In this paper the soy isolated protein was utilized as raw material and its optimum hydrolysis conditions of a alkaline proteolytic enzyme were determined by orthogonal design test. It was found that T= 55℃,pH8. 0, S= 10% and E/S= 4% are the preferred conditions and the hydrolysed protein thus obtained have a high yield with good solubility. The composition of essential amino acids of hydorlysed protein showed that Ile, Leu, Lys, Phe+Tyr, Thr and Val. have good blance closed to the reference patterns of NAS(1980) and FAO(1973) besides S-containing aminoacids.
【Key words】 Soy isolated protein; Proteolytic enzyme; Essential amino acids;
- 【文献出处】 食品与发酵工业 ,FOOD AND FERMENTATION INDUSTRIES , 编辑部邮箱 ,1994年05期
- 【分类号】TS214.2
- 【被引频次】105
- 【下载频次】589