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植物细胞离析酶的制备和应用

Preparation and Application of Maceration Enzyme from Aspergillus sp.

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【作者】 林开江袁康培王坤元陈兵董国强

【Author】 Lin Kaijiang Yuan Kangpei Wang Kunyuan Chen Bin Dong Guoqiang(Institute of Microbiology, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021)

【机构】 浙江省农业科学院微生物研究所浙江省农业科学院微生物研究所 杭州 310021杭州 310021杭州 310021

【摘要】 用Aspergillus sp.A-19菌经固体发酵研制成一种新的植物细胞离析酶(SeparataseZA-P)。其离析单细胞的酶活力平均为70767u/g,有效作用的pH在3.0—7.0,温度为20—45℃。发酵培养基配方是麸皮:桔皮粉:(NH42SO4(w/w)为100:100:0.63,料水比为1:2.0,培养适宜条件为25℃、60小时。

【Abstract】 Separatase ZA-P , a new enzyme for the maceration of plant cells, was prepared from Aspergillus sp. A-19 by solid fermentation. Its average activity to macerate the plant tissues into single cells was assayed as 70 767 u/g. The optimum pH for the maceration of plant cells was 3. 0-7, 0, and the optimum temperture was 20-45℃. The culture medium for the strain consisted of wheat bran, orange peel powder and ammonium sulphate in the ratio of 100 : 100 : 0. 63. The ratio of the dry materials to the water (w/v) was 2. 0 for the culture medium. The optimum culture condition was of 25℃ , and 60 hours.

  • 【文献出处】 生物工程学报 ,Chinese Journal of Biotechnology , 编辑部邮箱 ,1994年01期
  • 【分类号】TQ926
  • 【被引频次】12
  • 【下载频次】313
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