【摘要】 条斑紫菜的Ｒ－藻红蛋白（Ｒ－ＰＥ），在ＣＭ－５２柱上用含８ｍｏｌ／Ｌ脲的０．０２ｍｏｌ／Ｌ乙酸铵缓冲液（ｐＨ＝５．０５）洗脱，观察到３条色带，经吸收光谱测定表明，它们分别是α、β、γ亚基。用ＳＤＳ－ＰＡＧＥ测定的α、β和γ亚基分子量分别是１７．０ｋｄ，１８．０ｋｄ和３１．７ｋｄ。Ｒ－ＰＥ中亚基的摩尔比是６α：６β：１γ。条斑紫菜的Ｒ－ＰＥ最稳定的聚集态分子量是２２９ｋｄ。各亚基的发色团含量：α亚基含２个藻红胆素（ＰＥＢ），β亚基含１个ＰＥＢ和０．５个藻尿胆素（ＰＵＢ），γ亚基含２个ＰＥＢ和３个ＰＵＢ。结合Ｒ－ＰＥ和各亚基的氨基酸组成分析，条斑紫菜的Ｒ－ＰＥ亚基组成是（αβ）６γ。更多还原

【Abstract】 R-Phycocrythrin(R-PE) of prophyra yezoensis was applied to a column of CM-52, and washed with PH 5.05,0.02 M ammonium acetate buffer(containing SM Urea).Three fractions:the α、β and γ subunits were obtained separately,as determined by absorption spedra.The molecular weights ofα,β and γ subunits measured by SDS-PAGE were 17 kd,18 kd and 31.7 kd respectively. The molar ratio of subunits in R-PE were 6α:6β:1γ.The molecular weight of the most stable aggregate was 229 kd.The chromophore content of eaeh subunit was determined as: 2PEB for αsubunit; 1PEB and 0.5 PUB for β subunit and 2PEB and 3 PUB for γ subunit. Aecording to the results of the analysis of amlno acid compoaition of R-PE and eaeh individual subunit,the subunit composition of R-PE in prophyra yezoensis is(αβ)6 γ.更多还原