【摘要】 本文同凝胶色谱和琼脂糖－聚丙烯酸胺混合凝胶电泳法比较地研究了活性氧和病区黄腐酸（ＦＡ）对氨基多糖（ＧＡＧ）和蛋白多糖（ＰＧ）作用，并用恒ＰＨ值法研究了完整和受损的ＰＧ对矿化的影响。结果表明：１，黄嘌呤－黄嘌呤氧化酶体系（ＨＸ－ＸＯＤ）对ＧＡＧ无明显降解作用，只损伤ＰＧ的核心蛋白。２．Ｆｅ（ＩＩ）－ＥＤＴＡｓＨ－Ｈ２Ｏ２体系同时损伤ＰＧ和ＧＡＧ，并与Ｈ２Ｏ２浓度和作用时间正相关。３．ＦＡ不能直接降解ＧＡＧ和ＰＧ。４．ＰＧ能推延磷酸钙成核的诱导期，活性氧损伤后这种作用减弱。５．ＰＧ抑制磷酸盐的生长和聚集过程，受损的ＰＧ抑制能力减小，单位体积溶液中大颗粒的体积百分数下降。更多还原

【Abstract】 The actions of Fulvic acid (FA) and reactive oxygen species on the glycosamin oglycans (GAG) and proteoglycans(PG)were studied by means of column chromatog-raphy and p of yacrylamide-agarose composite gel electrophorsis. The effects of normal and injured proteoglyoan on mineralization were also studied by determining the utilization of hydroxyl ion in a PH-Stat. These results showed that: (1)HX-XOD system injured the core protein of PG, but caused no obvious degradation of GAGs; (2) Fe(II)-EDTA-H2O2 system damaged either GAGS or PGs in a time and H2O2 concentration-dependent manner; (3) FA did not degra de GAGsand PGs directly;(4)PGs delayed the induction time of calcium phosphate nucleation. Injured PGs had less effedt; (5)PGs inhibited the growth and aggregation of calcium phosphate cryStals. Injured PGs gave 4 low Percentage Of volume diffosion of large particles.更多还原