【摘要】 本文介绍一种用高效波相色谱（ＨＰＬＣ）同时测定血清中２４－脱氢胆固醇、７－烯胆甾烷醇、菜油甾醇、胆甾烷醇和β－谷甾醇的方法。血清加内标（６－氯豆甾醇）后用氢氧化钾醇溶液皂化，用正己烷提取其中的各种甾醇，将提出的甾醇衍生为苯氨基甲酸酯，用ＨＰＬＣ分离测定。本法样品处理简单，色谱分析时间短，批内和批间变异系数分别为２．２～３．８％和３．３～６．７％。本文也首次报告我国成年人血清非胆固醇甾醇水平，５种甾醇血清浓度的总和平均为１．５４ｍｇ／ｄｌ，是胆固醇的０．８６％。更多还原

【Abstract】 A simple method for simultaneous determination of desmosterol, lathosterol,campesterol, cholestanol and β-sitosterol in serum by high performance liquid chromatography (HPLC) is described. Serum containing internal standard (6-chlorostigmasterol) was treated with alcoholie potassium hydroxide and extracted with n-hexane. Sterols in the extracts were derivatized into phenylurethane and determined by HPLC, Within-and between-run CVs for 5 sterols were 2.2-3.8% and 3.3-6.7% respectively. Serum levels of non-cholesterol sterols inChinese adults were reported for the first time. The sum of the 5 sterols, averaged1 .54 mg/dl, accounted for about 0. 86 % of cholesterol.更多还原