【摘要】 揭示了吖啶橙的吸收光谱和荧光光谱对其浓度依赖性上的区域性特征，分析了测定溶酶体Ｈ￣＋转运时合理选用吖啶橙浓度及溶酶体用量的重要性、机理和原则，探讨了其与溶酶体的温育时间和Ｋ￣＋／Ｈ￣＋交换对测定Ｈ￣＋转运的明显影响。更多还原

【Abstract】 he different range characteristics of acridineorange in absorption and fluorescence is depen-dent on its concentrations.The importanceand mechanism as well as principles of choo-sing correct acridine orange concentration andrational amounts of lysosome in the assay ofprotons pumping are discussed. The obviousinfluences of the incubation time of acridineorange with lysosome and the K￣+/H￣+ ex-change upon the measurements of proton trans-port are studied.更多还原