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猪脑中提取高纯度神经节苷脂
Isolation and Extraction of Gangliosides withHigh Purity From the Pig Brain.
【摘要】 应用凝胶层析和离心液相层析法,从猪脑中提取高纯度神经节苷脂(Gls).按脂结合唾液酸(LBSA)计为30.1%,经硅胶薄层层析,580nm扫描结果表明含5种Gls,即:GMl为19.5%,GD3为13.8%,GDla为27.8%,GDlb为14.2%和GTlb为19.3%。
【Abstract】 Sephadex LH-20 and silica gel centrifugal liq-uid chromatography were applied to isolationand purification of gangliosides from the pigbrain. The highly purified gangliosides wereobtained. The concentration of lipid-boundsialic acid determined is 30.1% (W/W). Theresults determined by silica gel G-60 HPTLCand 580nm scanning were GMl 19. 5%. GD313.8%, GDla 27.8%, GD1b 14.2% andGTlb 19. 3%.
【关键词】 神经节苷脂(Gls);
猪脑;
离心液相层析;
纯化;
【Key words】 gangliosides. pig brain; centrifu-gal liquid chromatography; purification;
【Key words】 gangliosides. pig brain; centrifu-gal liquid chromatography; purification;
【基金】 北京市科委资助课题
- 【文献出处】 生物化学与生物物理进展 ,PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 编辑部邮箱 ,1994年05期
- 【分类号】Q546.03
- 【被引频次】15
- 【下载频次】302