【摘要】 为提高解离增强镧系荧光免疫分析（ＤＥＬＦＩＡ）的灵敏度或信／噪比，进行了一些重要的方法学研究．观察到了对于不同的铕量，荧光响应和信／噪比都随着增强液体积而明显地变化．对于确定的铕量，存在一个最佳体积，且铕量越小，其最佳体积也越小．实验中选择最佳体积是重要的．研究了增强液制备技术，发展了微滴定板条有效的清洗和干燥方法，使本底荧光明显降低．更多还原

【Abstract】 actors influencing the sensitivity, or the sig-nallnoise ratio.of dissociation enhanced lan-thaqide fluoroimmunoassay (DELFIAJ havebeen studied. The fluorescence responses andsignallnoise ratios for different europiumamount were shown to be changed with thevolume of enhancement solution.and therewas an optimum volume at a certain europiumamount.The smallest europium amount leadsto the smallest optimum volume.20/00 of thenet fluorescence intensity was increased by us-ing tinfoil reflection layer. Effective washingand drying methods of microtitration strips decreased background fluorescence have been de-veloped.更多还原