【摘要】 抗肿瘤药物阿霉素（ＡＤＭ）对心肌线粒体Ｆ１＿Ｆ＿０复合体呈现抑制而对Ｆ＿１－ＡＴＰａｓｅ无抑制，这表明ＡＤＭ可能是通过膜脂起作用的，适当浓度Ｍｇ￣（２＋）能降低ＡＤＭ对复合体的抑制。经￣（３１）Ｐ－ＮＭＲ和标记荧光探针ＮＢＤ－ＰＥ，ＤＰＨ，ＭＣ－５４０以及内源荧光等的测定，结果表明ＡＤＭ可能首先通过诱导Ｆ＿１Ｆ＿０膜脂形成非双层脂结构，继而影响了膜脂的堆积程度和流动性，进而引起Ｆ＿１Ｆ＿０复合体酶蛋白构象的改变，最终导致酶活力的降低。Ｍｇ￣（２＋）则可能由于与ＡＤＭ竞争与心磷脂的结合，而对ＡＤＭ引起Ｆ＿１Ｆ＿０的变化产生保护作用更多还原

【Abstract】 driamycin （ADM） is a widely used anticancerdrug, but the chronic cardiotoxicity severelylimits the use of it in the treatment of neoplas-tic disease. The experimental results obtainedfrom F₁,F₀-ATPase activity assay. si P-NMRspectra measurement. fluorescent probe NBD-PE detection. packing and fluidity ofmem-brane lipids and intrinsic fluorescence measure-ments can be summarized as follows: ADM in-duces the phase transition of mitochondrialmembrane lipids at first. as a consequence af-fecting on the lipid packing and fluidity of thelipid molecules and then influencing the con-formation of the F₁F₀-ATPase and finally re-sulting in the decreasing of the enzymatic ac-tivity. And Mg￣2+ can protect all the effects in-duced by ADM thus reducing the harmful ef-fect of ADM.更多还原