【摘要】 用ＨＢＶ　ＤＮＡ二聚体转染高度分化的人肝癌细胞株（ＨｅｐＧ２），筛选出具有分泌病毒抗原、核酸以及释放４２ｎｍ　Ｄａｎｅ颗粒能力的ｚ７－４细胞株。本实验结果支持３．５ＫｂｍＲＮＡ是前病毒基因，以它作模板可逆转录成１．６Ｋｂ（－）ｓｓＤＮＡ，再合成（＋）ｓｓＤＮＡ，最后装配成４．０Ｋｂ和３．２ＫｂｄｓＤＮＡ。在ＤＮＡ印迹法中位于４．０Ｋｂ（Ｄ１）和３．２Ｋｂ（Ｄ２）处是病毒的松驰环状双股ＤＮＡ（ＲＣＤＮＡ），而１．６Ｋｂ和１．２Ｋｂ处的２条ＤＮＡ带（Ｓ１和Ｓ２）均为单股ＤＮＡ，这些不同位置的ＤＮＡ带与病毒复制过程中ＤＮＡ的构型和片断大小不同有关。本实验未能发现２．０ＫｂｃｃｃＤＮＡ，但提示了负股ＤＮＡ５’端连接有蛋白质引物。更多还原

【Abstract】 Plasmid of HBV DNA dimer was transfected into a differentiated human hepatoma cell line(HepC2), and a stable HBV-producing cell line(z7-4) was set up which had capable of secreting HBcAg/HBeAg,viral nucleic acids and of releasing Dane-like particles.Our data showed that 3.5Kb pregenome RNA was the template for reversed transcription of 1.6Kb(-)ssDNA,then(-)ssDNA was biosynthesized and 4.0Kb and 3.2Kb dsHBV DNA were assembled. In Southern blot analysis of 1.5% agarose gel, the DNA bands at 4.0Kb(D1)and 3.2Kb(D2) released a circular HBV DNA(RCDNA),,and DNAbands at 1.6Kb(s1)and 1.2Kb(S2) belonged to a single-stranded HBV DNA. Several DNAbands were attributed to Various confor mations and sizes of intermediate HBV DNA.Although a 2.0Kb covalent closed circular HBV DNA(cccHBV DNA)was not found,a primer protein covalently combined with 5’ end of viral minus HBV DNA was identified.更多还原