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单克隆抗体反向间接血凝检测急性血吸虫病患者粪便虫源性抗原的效果

Detection of gun-associated cathodic antigen of schistosoma japonica in stool samples on acute schistosomiasis by McAb-RIHA

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【作者】 王勇王之怀胡万富汪昊尹晓梅刘晓明季虹吴维铎

【Author】 Wang Yong, et al Anhui Provincial Institute of Parasitic Diseases

【机构】 安徽省寄生虫病防治研究所安徽省寄生虫病防治研究所 芜湖 241000芜湖 241000

【摘要】 本文报告了McAb-RIHA法检测30例急性血吸虫病患者粪便虫源性抗原的结果。首次证实,急血患者粪便中存在日本血吸虫肠相关趋阴级抗原。McAb-RIHA法检测敏感性为90%,特异性为92.5%,与肝吸虫患者粪便的交叉反应率为10%。推测急血患者粪便中虫源性抗原可能是通过胆汁分泌途径排入肠道。文中对粪便中虫源性抗原检测在血吸虫免疫诊断上的意义进行了讨论。

【Abstract】 The gun-associated cathodic antigen of S.Japonica in stool samples of 30 cases of acute schistosomiasis patients, 20 cases of Clonorchiasis sinensis patient3 and 20 healthy persons were detected by McAb-RIHA respectively.The results showed that the positive rate was 90% on acute schistosomiasis, the specificity was 92.6%, the across-reaction rate with C.sinensis was 10%.So, it was discovered for the first time that the gut-associated cathodic antigen of S.japonica existed in the stool samples of acute schistosomiasis patients.This antigen in stool was deduced to be secreted through bile ducts.The immunodiagnostic significance about detection of the antigens originated from the S.japonica in stool was also discussed in this paper.

  • 【分类号】R532.21
  • 【被引频次】1
  • 【下载频次】11
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