【摘要】 建立人醛缩酶A单抗及其竞争性ELISA法测定血清ALD-A、最小检出量为5ng/m1,对ALD—B和C无交叉反应。测定60例正常人血清ALD—A为131±24ng/ml,上限176ng/ml,肝癌为554±163ng/ml,超过上限179ng/ml者95.5％(44/45),胃癌为482±241ng/ml,增高者75％(9/12)。本法灵敏度高、特异性强、方法简便、所用单抗可大量制备,能使ALD—A检测标准化,可用于临床肝癌诊断测定。更多还原

【Abstract】 Human Aldolase-A monoclonal antibody was preparated.Purified IgG was conjugated with horseradish peroxidase (HRP) .Using this prepared IgG-HRP conjugates, a highly sensitive competition ELISA was established to determine concentration! ALD -A in the serum. No cross reactions with human ALD -B, C were found. The serum levels of ALD-A in 60 normal subjects were 131±24 ng/ml. In contrast 65.5% of 45 patients with HCC (554 ±19gn3/ml) had ALD-A serum levels above the normal range 179 ng/ml(X±1.96SD). The ALD-A levels were increased in 75% of the patients with carcinomaof stomach (492±24lng/ml) . The determination of serum ALD-A by ELISA of human ALD - A McAb was Valuable method.更多还原