【摘要】 采用酶动力学及聚丙烯酰胺凝胶电泳方法研究了ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ、ＤＤＣ对酪氨酸酶的抑制作用。ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ为非竞争性抑制作用，随着抑制剂浓度增加，酶活性逐渐减弱。当ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ的浓度分别达到１．２ｍｍｏｌ／Ｌ１２。５ｍｍｏｌ／Ｌ１２．５ｍｍｏｌ／Ｌ时，酶活性的抑制率为１００％、５０％、２４％。ＤＤＣ对酪氨酸酶的抑制作用为竞争性抑制作用，其浓度达４ｍｍｏｌ／Ｌ时，酶活性１００％受到抑制。更多还原

【Abstract】 nhibition of tyrosinase activity by NaHSO3.KSCN. DDC and NaN3 was studied using en-zyme kinetics and polyacrylamide gel electroph analysis. DDC was found to be a competitive in-hibitor and NaHSO3. KSCN :NaN3 to be non-commpetitive inhibitors. When NaHSO3 and DDCused were 12 mmol/L and 4 mmol/L,tyrosinase activity was inhibited absolutely.12.5 mmol/LKSCN inhibited tyrosinas activity by 24%。12.5 mmol/L NaN3 inhibited tyrosinas activity by50%更多还原