【摘要】 本文以小鼠骨髓细胞（ＢＭＣ）和经条件化筛选的分化程度较一致的小鼠骨髓巨噬细胞（ＢＭＭ）为研究对象，摸索了用ＭＴＴ法测定人巨噬细胞集落刺激因子（ｈＭ－ＣＳＦ）生物活性的最佳条件。结果显示ＢＭＣ细胞的线性检测范围为６００～２５００ＣＦＵ／ｍｌ；ＢＭＭ细胞的线性检测范围明显改善，为１５０～１２５０ＣＦＵ／ｍｌ。该法的建立与改善提供了简便、可靠、适于检测大批量Ｍ－ＣＳＦ样品生物活性的方法。更多还原

【Abstract】 We developed a MTT colorimetry method to detect human macrophage colony stimulating factor(hM-CSF)using mouse bone marrow ceils(BMC) and bene marrow derived macropages(BMM), The detection range of lin-earity using BMC was 600 ～ 2500 CFU/ml,Compared with BMC, the linear range of detection using BMM cells wasgreatly improved which is 150～ 1250 CFU/ml; This indicaled that the established and the later improved MTT colorime-try for detecting hM-CSF is reli able, simple and appropriate for measuring large number of bM-CSF samples.更多还原