【摘要】 用化学偶联的双特异性抗体复合物－αＴＴ－αＩｇＭ通过体外实验。观察其抗原递呈能力。结果显示：αＴＴ－αＩｇＭ存在时，可使非特异性Ｂ细胞的抗原递呈效应显著提高，所需抗原浓度较不加αＴＴ－αＩｇＭ的对照组低１０００倍左右，与抗原特异性Ｂ细胞的抗原递呈效应相似。抑制试验显示：羊抗鼠ＩｇＭ，鼠ＩｇＭ能抑制αＴＴ－αＩｇＭ对非特异性Ｂ细胞抗原递呈作用的增强效应，表明Ｂ细胞膜ＩｇＭ在抗原递呈中起关键性作用。本研究结果表明：αＴＴ－αＩｇＭ的存在确能模拟抗原特异性Ｂ细胞的高效抗原递呈功能。更多还原

【Abstract】 By two independent assay(T cell proliferation &IL-2 secretion)the effect of bispecific antibody complexby chemical conjugated-aTT-aIgM on presentation of antigen was investigated,In the presence of aTT-aIgM.the abilityto present antigen of non-specific B cell was significantly enhanced and the antigen(TT) was presented at concentration10￣3-fold lower than that by non-specific B cell without aTT-aIgM or by M.but just the same as the antigen-specific Bcell.We also observed that the enhanced presentation was significantly inhibited by mouse IgM or anti-mouse-IgM. Theseresults indicated that high efficiency presentation of antigen-specific B cell can be mimicked by aTT-aIgM.更多还原