【摘要】 以杨树花药为外植体诱导单倍体念伤组织，进行耐氯化钠（ＮａＣｌ）和碱性盐（模仿天然盐碱土成份配成的几种盐的混合物）变异体的筛选。结果表明，不同的杨树品种对这两种选择剂的耐性是不同的，分化态愈伤组织比脱分化态愈伤组织显示出更强的抗性，不同的花药愈伤组织无性系间在抗盐能力上也存在差异。对再生植株的叶子和愈伤组织进行过氧化物同工酶分析，发现耐盐变异体与对照的酶谱是有差异的，前者比后者在相同带区常常多出一条谱带。更多还原

【Abstract】 election of variants tolerant to NaCl and alkaline salt was made from poplar anther callus in vitro culture. The results showed that different poplar species appeared different tolerance to the two screening agents. The calli in differentiation state showed stronger tolerance than that in dedifferentiation state.The differentiated calli of Populus simonii × P.nigra could tolerate 3% NaCl 0.75% alkaline salt. The regenerated plants of variants had transplanted to the soil. In comparing the maps of plantlets a clear difference was found be-tween salt-tolerant variants and controls.更多还原