【摘要】 对经多重多聚酶链反应（多重PCR）检测的在49例肺鳞癌中发现的7例人乳头瘤病毒（HPV）DNA阳性肿瘤组织又进行了生物素标记HPVDNA探针的原位杂交检测。结果5例呈杂交阳性，其中HPV11型阳性3例，HPV16型阳性1例，1例为HPV11和16型混合阳性。原位杂交显示HPVDNA大多位于凹空样肿瘤细胞的核内。分子生物学和肿瘤组织学研究表明HPV感染可能与部分肺鳞癌有较密切的关系。更多还原

【Abstract】 The human papilloma virus of 7 cases from 49 cases ill with squamous carcinoma of lung detected by multiple PCR (polymerase chain reactiion)was found positive for DNA and the tumors which were positive for HPV DNA were detected again by in situ hybridization with biotin-labelled probs. 5 cases were hybridization positive,and among them, 3 cases were positive for HPV type 11,on cases was HPV type 16,one cases was both type 11 and type 16,HPV DNA mainlylocated in the nuclei of koilocytotic-like tumor cells as illustrated by in situ hybridization. The study of molecular biology and histology of tumor indicates that there may be closer relation between the HPV infection and some of squamous carcimoma of lung.更多还原