【摘要】 雷蒙特红豆草１０ｄ龄无菌苗的下胚轴切段在附加２，４－Ｄ和ＫＴ各１ｍｇ／Ｌ的ＭＳ培养基上诱导愈伤组织。取培养３周后形成的愈伤组织进行酶解可获得大量原生质体。原生质体在修改的ＫＭ８Ｐ液体培养基中培养，第１０ｄ开始分裂，２周后形成小细胞团，４周时发育成大细胞团。第７周将小愈伤组织转于含０．５％琼指糖的固体培养基上，它们可以旺盛生长，成为有根、芽分化的胚性愈伤组织，转于分化培养基上发育成苗和小植株。无根苗转于附加有ＮＡＡ０．２ｍｇ／Ｌ、ＢＡ０．０５ｍｇ／Ｌ、蔗糖２％的ＭＳ培养基中生根。更多还原

【Abstract】 Calli were from hypocotyl explants cultured on medium containing 2,4-D 1 mg/L, KT 1 mg/L. The protoplasts were isolated from primary calli and cultured in modified liquid KM8P medium. The first division of the regenerated cell occured within 10 days.Small cell clusters were formed and then developed into the large cell colonies 2 and 4 weeks after the culture , respectively. When transferred to 0. 5% agarose solidified media , protocallus proliferated and turned into embryogenic calli with differentiated roots and buds.Plantlets and multiple shoots developing on calli were subcultured MS medium of BA 0. 5～1mg/L and NAA 0. l ～0. 5 mg/L. Roots were induced from shoots on MS medium containing NAA 0. 2 mg/L. BA 0. 05 mg/L and sucrose 20%.更多还原