【摘要】 本文以四种自动分析仪为例，在研究了各个测定参数后，建立果糖胺（ＧＳＰ）的自动分析方法。基本参数为：温度３７℃，波长５３０ｎｍ，速率法或二点法，延迟时间５～１０分钟，读数时间５分钟，标准曲线模式Ｌｏｇｉｔ／Ｌｏｇ或ｌｉｎｅａｒ，样本／试剂比１：２０．试剂为０．２５ｍｍｏｌ／ＬＮＢＴ，标准用１－脱氧吗啉果糖（ＤＭＦ）。本法的线性范围达６ｍｍｏｌ／Ｌ，并具有良好的精密度与准确度，批内ＣＶ＜２．３％，批间ＣＶ＜４％，平均回收率１０５％。以本法测定的正常值范围为１．０７～１．８３ｍｍｏｌ／Ｌ。更多还原

【Abstract】 Researched the measurement parameters, we established the autoanalysis method of serum GSP in four types of analyzors. The base parameter were: T 37℃, λ530 nm, reaction mode kinetic, delay time 5～10min, read time 5 min, calibration mode logit/log or linear, volum of sample to reagent 1 : 20. the concentration of NBT was 0.25 mmol / L and 1-deoxy-1-morpholinofructose(DMF) was used as calibrator. The linearity of this determination was 6 mmol/L. The precision and accuracy was good,within-and between-batch CVs < 2.3 and <4% , respectively, the mean rate of recovery was 105% The normal range of this assay was 1.07-1.83 mmol/L.更多还原